Long non?coding RNA MEG3 inhibits cell migration and invasion of non?small cell lung cancer cells by regulating the miR?21?5p/PTEN axis

Long non?coding RNAs (lncRNAs) are involved in the occurrence and progression of numerous types cancer. The aim present study was to evaluate effect lncRNA maternally expressed gene 3 (MEG3) on migration invasion non?small cell lung cancer (NSCLC) H1299 PC9 cells. Reverse transcription?quantitative (RT?q)PCR analysis showed that MEG3 downregulated NSCLC Additionally, bioinformatics indicated sponges microRNA (miR)?21?5p; miR?21?5p predicted target phosphatase tensin homolog (PTEN) 3'?untranslated region sequence. overexpression led suppression PTEN upregulation cells, as detected by RT?qPCR. Subsequently, western blot confirmed enhanced expression levels inhibited PI3K/AKT signaling pathway These effects were attenuated miR?21?5p. Dual luciferase assay supported sponging validated direct interaction between PTEN. Furthermore, Transwell demonstrated had an inhibitory invasion. also mediated epithelial?to?mesenchymal transition significantly enhancing E?cadherin decreasing N?cadherin, Vimentin matrix metalloprotein 9 analysis. changes partially reversed mimic. results acted a tumor suppressor via miR?21?5p, which, turn, PTEN, part pathway. have suggested potential novel therapeutic for treatment.